FIGURE

Fig. 3

ID

ZDB-FIG-230302-16

Publication

Kamstra et al., 2022 - Leptin regulates glucose homeostasis via the canonical Wnt pathway in the zebrafish

Other Figures

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Fig. 3

Wnt pathway activation by leptin in Tg(7xTCFXla. Siam:nlsmCherry)^ia5 larvae. (A) 5 dpf Tg(7xTCFXla. Siam:nlsm Cherry)^ia5 larvae treated with vehicle (Cortland salt solution). Left: bright field image, with anatomical landmarks encircled; Middle: Epifluorescence image, with white arrow indicating teeth protrusion; Right: Magnification of yellow box in middle image, with hypothalamus and heart encircled. Scale bar = 500 μM. (B) 5 dpf Tg(7xTCFXla. Siam:nlsm Cherry)^ia5 larvae treated with 100 nM recombinant leptin for 2 h. (C) 5 dpf CRISPR-mediated leptin receptor-deficient Tg(7xTCFXla. Siam:nlsm Cherry)^ia5 larvae treated with 100 nM recombinant leptin for 2 h. (D) 5 dpf CRISPR-mediated leptin receptor-deficient Tg(7xTCFXla. Siam:nlsm Cherry)^ia5 larvae treated with 10 μM LiCl for 2 h. (E) Fluorescence intensity in the hypothalamus of differentially treated 5 dpf Tg(7xTCF-Xla. Siam:nlsmCherry)^ia5 larvae. A–B = p < .05, one-way ANOVA. (F) Fluorescence intensity in the heart of differentially treated 5 dpf Tg(7xTCF-Xla. Siam:nlsmCherry)^ia5 larvae. A–B = p < .05, one-way ANOVA

Expression Data

Gene:	mCherry
Fish:	ia5Tg ia5Tg + CRISPR2-lepr
Conditions:	chemical treatment by injection: Leptin chemical treatment by environment: lithium chloride
Knockdown Reagent:	CRISPR2-lepr
Anatomical Terms:	heart hypothalamus
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ia5Tg ia5Tg + CRISPR2-lepr
Conditions:	chemical treatment by injection: Leptin chemical treatment by environment: lithium chloride
Knockdown Reagent:	CRISPR2-lepr
Observed In:	heart heart canonical Wnt signaling pathway hypothalamus hypothalamus canonical Wnt signaling pathway
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ FASEB J.