Fig. 5

Zebrafish histone H2A inhibits the effects mediated by TBK1. (A) Effect of zebrafish histone H2A on the TBK1-mediated antiviral activity. (B) The confirmation of zebrafish histone H2A expression in the histone H2A-overexpressed ZF4 cells with SVCV infection. (C) Effect of zebrafish histone H2A on the expression of SVCV-N gene mediated by TBK1. (D) Effect of zebrafish histone H2A on the expression of SVCV-G gene mediated by TBK1. (E) Effect of zebrafish histone H2A on the expression of IFN1 mediated by TBK1. (F) Effect of zebrafish histone H2A on the expression of IFN3 mediated by TBK1. (G) Effect of zebrafish histone H2A on the expression of PKZ mediated by TBK1. (H) Effect of zebrafish histone H2A on the expression of RSAD2 mediated by TBK1. (I) Effect of zebrafish histone H2A on the expression of MxA mediated by TBK1. (J) Effect of zebrafish histone H2A on the expression of MxB mediated by TBK1. (K) Effect of zebrafish histone H2A on the expression of MxC mediated by TBK1. (L) Effect of zebrafish histone H2A on the expression of MxE mediated by TBK1. For (A–L) ZF4 cells were transfected with the indicated plasmids. At 36 h post-transfection, these cells were used for SVCV infection at an MOI of 1. At 48 hpi, the supernatants of transfected cells were collected for the determination of virus titers. The cell pellets of transfected cells were collected and used for qRT-PCR. Data represented means ± SEM (n = 3), and were tested for statistical significance. *p < 0.05; **p < 0.01. The asterisk above the error bars indicates statistical significance using the group microinjected with empty plasmid as the control group. The asterisk above the bracket indicates statistical significance between the two groups connected by the bracket.