FIGURE
Figure 4
- ID
- ZDB-FIG-230122-32
- Publication
- Keseroglu et al., 2023 - Using single-molecule fluorescence in situ hybridization and immunohistochemistry to count RNA molecules in single cells in zebrafish embryos
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Figure 4
Surface creation and masking image (A) Stitch ND2 raw image. One of the (B) Converted stitched file to Imaris format. (C) Select tissue format in every 5 slices using manual surface creation. (D and E) Created surface to mask DAPI (nucleus) and FITC (membrane) channels. (F) Use “Mask All” in the “Tab Edit”. Set voxel intensity outside surface to zero “0”. (G) Masked tissue. (H and I) (H) Use “free rotate” window to make image anterior-posterior view (I) from left to right. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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