Fig. 7

The rose diagrams in a-d represent the angle of migration with respect to the ML axis. Angles are divided along the AP axis, with cells adjacent to somite 1 in red, cells adjacent to somite 2 in yellow, cells adjacent to somite 3 in green and cells adjacent to somite 4 in blue. (a) Wildtype embryos show a directed migration, with different angles of migration for somites 1-4. (b) Tbx5a knock-down embryos display equivalent angles of migration across the AP axis. (c) Tbx5b knock-down embryos show wt-like angles of migration along the AP axis, but randomized ML movements. (d) Double knock-down embryos display both AP and ML changes to the angles of cell migration across all positions. (e-f) Angle of xCentrin::RFP expression with respect to the nucleus in the cells of the wt fin field. (e) In 18 hpf embryos, the angle of xCentrin::RFP expression with respect to the nucleus displayed no significant bias in angle. (f) At 22 hpf, the angle of xCentrin::RFP with respect to the nucleus displayed no significant bias in angle.