Fig. 1

Tbx5 knock-down embryos display heart and pectoral fin defects. (a-a’’’) Arrowheads point to position of heart. Views are lateral with anterior to the left. Scale bar=250μm. (a) Normal wt heart development. (a’) Tbx5a-knock-down embryos display edema and defects in heart looping. (a’’) Tbx5b-knock-down embryos display edema and defects in heart looping. (a’’’) Double knock-down embryos have linear hearts and edema, (b-b’’’) Arrowheads point to normal position of pectoral fins. Views are from dorsal aspect with anterior to the top. Scale bar=100μm. (b) Wt embryo showing both fins at 3 days post-fertilization (dpf). (b’) Tbx5a knock-down embryos lack pectoral fins, (b’’) Tbx5b knock-down embryos display small, misshapen fins. (b’’’) Tbx5a/Tbx5b double knock-down embryos do not form pectoral fins. Panels a, a’’, b and b’’ reproduced from Boyle Anderson and Ho (2018).