FIGURE

Fig. 1

ID

ZDB-FIG-221207-13

Publication

Jia et al., 2022 - Tulp1 deficiency causes early-onset retinal degeneration through affecting ciliogenesis and activating ferroptosis in zebrafish

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Fig. 1

Generation of the tulp1a^−/− and tulp1b^−/− zebrafish lines.

A The gene structure and CRISPR/Cas9 target sites are shown. The numbers represent exons. B DNA sequencing showing the tulp1a mutant line (tulp1a^−/−) (c.115_118delGGTG), and tulp1b mutant line (tulp1b^−/−) (c.591delC). C Decreased expression of tulp1a or tulp1b was detected by qRT-PCR in, respectively, tulp1a^−/−, tulp1b^−/−, and in tulp1-dKO zebrafish at 4 dpf. 18s-rRNA was used as an endogenous control (n = 3). Mean ± SD. **P < 0.01. ****P < 0.0001. D, E Whole-mount in situ hybridization showing the expression pattern of tulp1a and tulp1b in wt and tulp1-dKO zebrafish. (n = 20). Scale bar: 50 µm. F The protein level of Tulp1 was undetectable at 4 mpf (n = 3). dpf: day post-fertilization; mpf: month post-fertilization.

Expression Data

Genes:	tulp1a tulp1b
Antibody:	Ab1-tulp1
Fish:	WT tulp1a^hzu18/hzu18 tulp1b^hzu19/hzu19 tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19
Anatomical Terms:	epiphysis eye head retina whole organism
Stage Range:	1-cell to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tulp1a^hzu18/hzu18 tulp1b^hzu19/hzu19 tulp1a^hzu18/hzu18; tulp1b^hzu19/hzu19
Observed In:	eye head retina
Stage Range:	Long-pec to Adult

Phenotype Detail

Acknowledgments

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