IMAGE

Fig. 1

ID

ZDB-IMAGE-221207-6

Genes

Antibodies

Ab1-tulp1

Source

Figures for Jia et al., 2022

Image

Figure Caption

Fig. 1

Generation of the tulp1a^−/− and tulp1b^−/− zebrafish lines.

A The gene structure and CRISPR/Cas9 target sites are shown. The numbers represent exons. B DNA sequencing showing the tulp1a mutant line (tulp1a^−/−) (c.115_118delGGTG), and tulp1b mutant line (tulp1b^−/−) (c.591delC). C Decreased expression of tulp1a or tulp1b was detected by qRT-PCR in, respectively, tulp1a^−/−, tulp1b^−/−, and in tulp1-dKO zebrafish at 4 dpf. 18s-rRNA was used as an endogenous control (n = 3). Mean ± SD. **P < 0.01. ****P < 0.0001. D, E Whole-mount in situ hybridization showing the expression pattern of tulp1a and tulp1b in wt and tulp1-dKO zebrafish. (n = 20). Scale bar: 50 µm. F The protein level of Tulp1 was undetectable at 4 mpf (n = 3). dpf: day post-fertilization; mpf: month post-fertilization.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Death Dis.