Fig. 5
- ID
- ZDB-FIG-221122-7
- Publication
- Basnakova et al., 2021 - The habenula clock influences response to a stressor
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Fig. 5. The effects of the alarm substance Schreckstoff on Tg(gng8:GAL4, UAS:EGFP-Δclocka) fish. (A–D) Acute effects in adult fish (N = 12). (A) Schematic diagram of the experiment. (B) Speed distribution in fish before and after exposure to the alarm substance, where the substance remained in the tank after delivery. (C–D) Time spent darting (C), restricted to the tank base (D) in the presence of the alarm substance. As indicated by the paired mean difference plots (lower plot), fish with and without expression of truncated clocka behave similarly. (E–G) Post-exposure behaviour in adult fish (N = 8). (E) Schematic diagram of the experiment. (F) Speed distribution in a novel tank containing clean water, after exposure to alarm substance or clean water. (G) Distance traversed in a novel tank containing clean water, at different times after exposure to the alarm substance. (H–J) Post-exposure behaviour in 2 week-old zebrafish. (H) Schematic diagram of the experiment. (I) Percentage of time in the dark area of a tank, after 10 min of exposure to embryo water. (J) Percentage of time spent in the dark side after exposure to the alarm substance. Tg(gng8:GAL4, UAS:EGFP-Δclocka) fish spend more time in the dark side, with a Cohen's D of 1.2. |