FIGURE

Fig. 4

ID

ZDB-FIG-221121-42

Publication

Lin et al., 2021 - Sirt1 promotes tissue regeneration in zebrafish through regulating the mitochondrial unfolded protein response

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Fig. 4

Figure 4. Sirt1 inhibition impairs adult fin regeneration via reduction of the UPR^mt

(A) Representative images (left) and quantification (right) show that inhibiting Sirt1 by adding NAM to fish water impairs fin regeneration in adult zebrafish.

(B) Representative in situ hybridization images show that NAM treatment reduces hspd1 expression levels in the blastema of an injured adult fin.

(C) The representative images (left) and quantification (right) show that incubation of injured hspd1 mutant fish at a semi-permissive temperature in conjunction with a low concentration of NAM results in significant inhibition of fin regeneration.

(D) Quantification of regeneration growth shows that sirt1 mutant larval fish exhibit a fin regeneration defect when treated with doxycycline. Each dot represents a fin regeneration measurement from one fish. Error bars indicate standard deviation. Student’s t test was performed to determine statistical significance. ∗p < 0.05.).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TU hspd1^zp7/zp7 (AB/TU) sirt1^wcm21/wcm21 (AB/TU)
Conditions:	amputation: caudal fin amputation: caudal fin, temperature exposure amputation: caudal fin, chemical treatment by environment: nicotinamide, temperature exposure amputation: caudal fin, chemical treatment by environment: doxycycline amputation: caudal fin, chemical treatment by environment: nicotinamide
Observed In:	blastema fin regeneration
Stage:	Adult

Phenotype Detail

Acknowledgments

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