Fig. 1
- ID
- ZDB-FIG-221111-1
- Publication
- Lam et al., 2021 - Identification of a wide spectrum of ciliary gene mutations in nonsyndromic biliary atresia patients implicates ciliary dysfunction as a novel disease mechanism
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Fig. 1. Absence of cilia staining in the bile ducts of BA livers of the two patients harboring LOF mutations in the ciliary genes. Co-immunofluorescence staining for α-Tubulin (TUB; red) and Pericentrin (PCNT; green) was performed on liver sections of Non-BA control (Non-BA CTRL; top left), BA patients with no ciliary gene mutation (Non-mutant BA; bottom left) and BA patients with ciliary gene mutation (Mutant BA; BA650C and BA634C; top and bottom right). α-Tubulin immuno-reactivity was co-localised with PCNT at the luminal surface of the bile ducts of Non-BA CTRL and Non-mutant BA livers. In contrast, only immuno-staining for PCNT were detected but expression of α-Tubulin were absent in BA650C and BA634C patients’ bile ducts. Nuclei were stained with DAPI. Representative photos were shown for comparison. Highlighted regions were magnified as shown in inset. Number of patients examined in each group was indicated as “n”. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article). |