FIGURE

Fig. 7

ID

ZDB-FIG-221018-61

Publication

Huang et al., 2022 - Intrinsic myocardial defects underlie an Rbfox-deficient zebrafish model of hypoplastic left heart syndrome

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Fig. 7

Injection of human RBFOX2 mRNA restores cardiovascular development in rbfox-deficient zebrafish, while HLHS-linked RBFOX2 variants fail to rescue.

a Brightfield images of 72 hpf CTRL, DKO, and DKO embryos injected with wildtype human RBFOX2 (hRBFOX2) mRNA. b Brightfield images with fluorescent overlay of Tg(myl7:GFP) reporter signal in hearts of 72 hpf CTRL, DKO, and DKO embryos injected with wildtype hRBFOX2 mRNA. c Dot plot showing % FAC of 72 hpf ventricles in the following cohorts: uninjected CTRL, uninjected DKO, DKO+ wildtype hRBFOX2, DKO+ RBFOX2-v1, v-2, or v-3 mRNA where hRBFOX2 harbors a variant (v) previously discovered in patients with HLHS. Sample sizes and statistics: (c) uninjected CTRL, n = 26; uninjected DKO, n = 23; DKO+ wildtype human RBFOX2, n = 25; DKO + hRBFOX2 v1, v2, or v3 (n = 8 per group). Each dot represents 1 embryo. Data are presented as mean values +/− one SD. Statistical significance was determined by ordinary one-way ANOVA Dunnet’s multiple comparisons test. P values are shown. Source data are provided as a Source Data file. Scale bars: 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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