FIGURE

Fig. 4

ID

ZDB-FIG-220920-72

Publication

Tian et al., 2022 - WRN promotes bone development and growth by unwinding SHOX-G-quadruplexes via its helicase activity in Werner Syndrome

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Fig. 4

WRN helicase is essential for chondrogenesis.

a–d. qRT-PCR measurement of different WRN expressions on day 14 in hESCs (a, b) and hMSCs (c, d). (Vector, full-length WRN, X-WRN (E84A), K-WRN (K57M), R-WRN (R993A), F-WRN (F1037A)). N = 3 biological independent experiments. Data are presented as the mean ± S.D. Statistical analysis was performed using two-tailed unpaired Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001. e Representative immunofluorescent staining of 3 independent experiments between the CTR and KD-WRN group on day 14 in hESCs. SOX9 and COL2A1 were examined. Scale bar = 50 µm. f Representative calcein staining of three independent experiments to examine the bone formation after microinjection of different human WRN mRNA in one-cell stage and checked at 7 dpf. Scale bar = 100 µm. g, h Representative WISH analysis of 3 independent experiments to examine the expression of sox9a and col2a1a after microinjection of different human WRN mRNA in one-cell stage and checked on 7 dpf. Scale bar = 50 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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