Fig. 7

Fig. 7. Inhibition of arg1/2 could not further down-regulated the reduction in neutrophils migration and cytokines level caused by mAChR activation. (A) Neutrophils were photographed with a fluorescence microscope. The caudal fin was not injured in the right picture, and the caudal fin was injured in the left picture. Neutrophils migration was reduced after treatment with bethanechol (10 μM) in the statistical area, and treated with bethanechol (10 μM) and CB-1158 (10 μM), neutrophils migration in the statistical area was unchanged compared to the bethanechol (10 μM) treatment alone. (B) Model of caudal fin injury: caudal fins of zebrafish treated or untreated with bethanechol (10 μM), bethanechol (10 μM) and CB-1158 (10 μM) were cut with a scalpel blade (white rectangles indicate statistical areas). (C) When no damage to the caudal fin, bethanechol (10 μM) treatment, bethanechol (10 μM) and CB-1158 (10 μM) treatment showed no difference in the number of neutrophils in the statistical area (white rectangles indicate statistical area). (D) Treatment with bethanechol (10 μM), bethanechol (10 μM) and CB-1158 (10 μM) in LPS induced inflammation model to detect the expression levels of tnf-α, il-1β, il-6 and il-8 (n = 30). (*P < 0.05，** P < 0.01, ***P < 0.001, ****P < 0.0001, one-way ANOVA analysis, LSD for port-hoc test).