FIGURE

Fig. 4

ID

ZDB-FIG-220912-164

Publication

Zhuang et al., 2022 - Histone Deacetylases Cooperate with NF-κB to Support the Immediate Migratory Response after Zebrafish Pronephros Injury

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Fig. 4

hdac6 and hdac8 are necessary for the pronephros repair process. (A) In situ hybridization revealed an upregulation of hdac6 (upper panel) and hdac8 (lower panel) two hours after laser-induced injury. The arrow points to the injury site. (B) Depletion of zebrafish hdac6 by translation- (TBM) blocking morpholino oligonucleotides (MO) significantly reduced the percentage of embryos that repaired the pronephric tubule (Fisher’s exact test, two-tailed). Co-injection of hdac6 mRNA rescued the hdac6 TBM pronephros repair phenotype. (C) Depletion of hdac8 with translation-blocking morpholino oligonucleotides significantly reduced number of repaired pronephri. The group size is shown in brackets. Individual experiments with mean and standard deviation for each group are displayed. Significance was calculated with Fisher’s exact test (two-tailed).

Expression Data

Genes:	hdac6 hdac8
Fish:	li1Tg; uf1Tg
Condition:	light ablation: pronephros
Anatomical Term:	pronephros
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	li1Tg; uf1Tg li1Tg; uf1Tg + MO1-hdac6 li1Tg; uf1Tg + MO1-hdac8
Condition:	light ablation: pronephros
Knockdown Reagents:	MO1-hdac6 MO1-hdac8
Observed In:	pronephros pronephros wound healing
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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