Fig. 1

(A): Phenotype of wild-type, nop56^+/− and nop56^−/− between 24 and 120 hpf. At 24 hpf, nop56^−/− show statistically significant reduced head size, smaller eyes and smaller. At 48 hpf, statistically significant reduced body length can be also observed in nop56^−/− embryos, as well as swollen yolk (in 100% of nop56^−/− embryos) and curved body shape in (40% of the nop56^−/− embryos). At 72 hpf, in addition to the previous features, there is cardiac edema in 77% of the embryos. In addition, at 96 and 120 hpf, there is total absence of swimming bladder in all nop56^−/− embryos, curved body shape (in 50% of nop56^−/− larvae) and a cardiac edema (in 88% of the nop56^−/− larvae at 96 hpf and 100% at 120 hpf). For these parameters, no statistically significant differences were observed between wild type and nop56^+/−. Statistically significant data in the graphs are indicated with a *. Scale bar: 1000 μm. (B) Kaplan–Meier survival graph for wild-type (n = 43), nop56^+/− (n = 75) and nop56^−/− (n = 44) zebrafish, revealing significantly different survivals for the nop56^−/− embryos p < 0.0001.