Fig. 10

Distribution of Otpa-positive cells compared to islet1-GFP cells in the adult zebrafish brain. Five transverse sections levels in DAPI stain (a-e), Otpa immunostain (a1-e1), GFP immunostain (a2-e2) and merged pictures (a3-e3). (a/b-a3/b3): telencephalon and preoptic region. Note one double-labeled cell in the supraoptic ventral telencephalic nucleus (Vs), yellow arrow in (a3) and two further ventricularly located double-labeled cells in (b3) (yellow arrows; see text). (c/d/e) to (c3/d3/e3): three levels of posterior tuberculum and hypothalamus. Several double-labeled cells sit in the ventral periventricular hypothalamic zone (Hv), yellow arrows in (c3). Note single labeled Otpa and islet1-GFP cells in the intermediate hypothalamic nucleus (IN), white arrows pointing dorsally in (d1-d3), and single labeled islet1-GFP cells in the ventral periventricular hypothalamic zone (ventrally pointing white arrows in d3). See text for more details. Scale bar = 250 µm. Abbreviations: Ac, anterior commissure; ATN, anterior tuberal nucleus; Dm, medial zone of dorsal telencephalon; ENv, ventral entopeduncular nucleus; Ha, habenula; Hc-a, caudal zone of periventricular hypothalamus in front of posterior recess; Hd/Hv, dorsal/ventral zone of periventricular hypothalamus; IN, intermediate hypothalamic nucleus; PM, magnocellular preoptic nucleus; PPa/PPp, anterior/posterior parvocellular preoptic nucleus; PTN, posterior tuberal nucleus; PVO, paraventricular organ; SC, suprachiasmatic nucleus; TeO, optic tectum; TPp, periventricular nucleus of posterior tuberculum; Vi/Vs, intermediate/supracommissural nucleus of ventral telencephalon