FIGURE

Fig. 4

ID

ZDB-FIG-220720-110

Publication

Meier et al., 2022 - Granulocyte Colony-Stimulating Factor Mediated Regulation of Early Myeloid Cells in Zebrafish

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Fig. 4

Role of alternative G-CSFR ligands in emergency myelopoiesis. (A–C) qRT ${}^{2}$ ${}^{2}$ -PCR analysis of wild-type embryos (A) or those injected with scr, csf3a, csf3b or csf3a and csf3b (csf3a/b) Mo without (-) or with (+) LPS treatment as indicated, for expression of csf3a and csf3b (A), mpo (B) or lyz (C) showing mean and S.E.M (n = 3). (D–I) Fluorescent microscopy images of representative Tg (lyz::DsRed) embryos injected with either scr Mo (D,E), csf3a Mo (F,G) or csf3a/b Mo (H,I), either without (-) (D,F,H) or with (+) (E,G,I) LPS treatment. (J) Quantitation of lyz+ cells in Tg (lyz::DsRed) embryos injected with the indicated morpholinos either without (-) with (+) LPS treatment showing individual embryos as well as mean and S.E.M.. For panels A–C and J statistical significance is indicated (*: p $<$ $<$ 0.05; **: p $<$ $<$ 0.001; ***: p $<$ $<$ 0.0001; ns, not significant). For panels B and C, black indicates relative to scr Mo -LPS and red indicates relative to scr Mo +LPS.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Biosci (Landmark Ed)