FIGURE

Fig. 6

ID

ZDB-FIG-220715-54

Publication

Berger et al., 2022 - Mob4-dependent STRIPAK involves the chaperonin TRiC to coordinate myofibril and microtubule network growth

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Fig. 6

Loss of mob4 function compromises neuronal connectivity.

(A) At 3 dpf, toluidine blue-stained sections displayed pyknotic nuclei (arrowhead) dispersed throughout the retina and tectum of mob4^geh homozygotes, not siblings (n = 4 per genotype). (B) Abundant apoptosis within the retina and tectum of 3-dpf-old mob4^geh homozygotes was detected by TUNEL assay (n = 8 per genotype). (C) In representative ventral views (Z-stack), the optic chiasm (asterisk) was highlighted by Tg(ath7:GFP) within 3-dpf-old siblings but not mob4^geh homozygotes (n = 3 per genotype). (D) In representative Z-stacks, axons of retinal ganglion cell marked by Tg(ath7:GFP) project contralaterally via the optic chiasm (asterisk) from the retina (arrowhead) onto the tectum (arrow) of 3-dpf-old siblings (n = 4). In contrast, axons were not formed by retinal ganglion cells (arrowhead) of mob4^geh homozygotes (n = 4). (E) In Z-stacks of 3-dpf-old larvae, antibodies against acetylated α-tubulin revealed defective neurite formation within the tectum of mob4^geh homozygotes. Boxed areas are shown in higher magnification (n = 3 per genotype). Scale bar sizes are indicated.

Expression Data

Gene:	GFP
Antibody:	Ab1-tuba
Fish:	TU mob4^pc43/pc43 (TU) rw021Tg mob4^pc43/pc43; rw021Tg
Anatomical Terms:	cranial nerve II intertectal commissure optic tectum optic tectum neuron projection retinal ganglion cell axon retinal ganglion cell layer
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mob4^pc43/pc43 (TU) mob4^pc43/pc43; rw021Tg
Observed In:	cranial nerve II cranial nerve II axon extension intertectal commissure optic tectum optic tectum apoptotic process optic tectum axon extension retina apoptotic process retinal ganglion cell axon extension visual system
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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