Fig. 2

(A-C) Schematics of the wild-type cacna2d3 allele (A), the cacna2d3^sa16189 mutant allele (B), and the cacna2d3^sa16051 mutant allele (C). (D) Mean acoustic startle responsiveness to each stimulus in the habituation assay. Animals were generated from a cacna2d3^sa16189/+ incross. Wild-type larvae are shown in blue, cacna2d3^sa16189/+ heterozygotes are shown in black, and homozygous cacna2d3^sa16189 mutants are shown in red. (E) Mean acoustic startle habituation percentage. (F) Mean acoustic startle responsiveness to each stimulus of the habituation assay. Animals were generated from a cacna2d3^sa16051/+ incross. Wild-type larvae are shown in blue, cacna2d3^sa16051/+ heterozygotes are shown in black, and homozygous cacna2d3^sa16051 mutants are shown in red. (G) Mean acoustic startle habituation percentage. (E, G) ***p<0.001, ****p<0.0001, one-way ANOVA with Dunnett’s multiple comparison test. (H) Mean acoustic startle responsiveness to each stimulus of the habituation assay. Animals were generated from a cross between cacna2d3^p177 mutants with cacna2d3^sa16189/+ heterozygotes. cacna2d3^p177/+ larvae are shown in blue and cacna2d3^p177/cacna2d3^sa16189 trans-heterozygotes are shown in red. (I) Mean acoustic startle habituation percentage. (J) Mean acoustic startle responsiveness to each stimulus of the habituation assay. Animals were generated from a cross between cacna2d3^p177 mutants and cacna2d3^sa16051/+ heterozygotes. cacna2d3^p177/+ larvae are shown in blue and cacna2d3^p177/cacna2d3^sa16051 trans-heterozygotes are shown in red. (K) Mean acoustic startle habituation percentage. (I, K) ***p<0.001, ****p<0.0001, Mann-Whitney test versus wild-type. For all panels, the number of larvae analyzed is shown below each bar. Error bars indicate SEM.