FIGURE

Fig. 1

ID

ZDB-FIG-220627-49

Publication

Xue et al., 2022 - HOX epimutations driven by maternal SMCHD1/LRIF1 haploinsufficiency trigger homeotic transformations in genetically wildtype offspring

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Fig. 1

Generation of MZ smchd1 null zebrafish.

a Three loss-of-function (LoF) alleles in zebrafish were generated by CRISPR/Cas9 injections targeting smchd1. b Endogenous spatial-temporal expression pattern of smchd1 in zebrafish embryos as determined by qPCR and WISH. n = 3 biological samples (qPCR), 3 independent experiments with at least 20 embryos each (WISH). Data are presented as mean values ± SD. c Maternal zygotic (MZ) smchd1^lof embryos lack smchd1 mRNA (qPCR and WISH) and protein (Western blot), suggestive of total nonsense-mediated decay. Scale bar = 200 μm. n = 2 biological samples (qPCR, Western blot), 3 independent experiments with at least 20 embryos each (WISH). d MZ smchd1^lof zebrafish are viable and fertile, and do not model arhinia at 3 months. Scale bar = 1 mm.

Expression Data

Gene:	smchd1
Antibody:	Ab3-smchd1
Fish:	AB smchd1^ns301/ns301 (AB) smchd1^ns302/ns302 (AB) smchd1^ns303/ns303 (AB)
Anatomical Terms:	head lateral plate mesoderm whole organism
Stage Range:	2-cell to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	smchd1^ns301/ns301 (AB) smchd1^ns302/ns302 (AB) smchd1^ns303/ns303 (AB)
Observed In:	whole organism
Stage Range:	2-cell to Adult

Phenotype Detail

Acknowledgments

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