IMAGE

Fig. 1

ID

ZDB-IMAGE-220627-39

Genes

smchd1

Antibodies

Ab3-smchd1

Source

Figures for Xue et al., 2022

Image

Figure Caption

Fig. 1

Generation of MZ smchd1 null zebrafish.

a Three loss-of-function (LoF) alleles in zebrafish were generated by CRISPR/Cas9 injections targeting smchd1. b Endogenous spatial-temporal expression pattern of smchd1 in zebrafish embryos as determined by qPCR and WISH. n = 3 biological samples (qPCR), 3 independent experiments with at least 20 embryos each (WISH). Data are presented as mean values ± SD. c Maternal zygotic (MZ) smchd1^lof embryos lack smchd1 mRNA (qPCR and WISH) and protein (Western blot), suggestive of total nonsense-mediated decay. Scale bar = 200 μm. n = 2 biological samples (qPCR, Western blot), 3 independent experiments with at least 20 embryos each (WISH). d MZ smchd1^lof zebrafish are viable and fertile, and do not model arhinia at 3 months. Scale bar = 1 mm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.