FIGURE

Fig. 2

ID

ZDB-FIG-220607-31

Publication

Varela et al., 2022 - Cdkl5 mutant zebrafish shows skeletal and neuronal alterations mimicking human CDKL5 deficiency disorder

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Characterization of cdkl5^sa21938 mutant at initial larval stages. (A) Sequencing chromatograms showing the zebrafish genotypes for cdkl5. Arrows indicate the mutation site. (B) Representative lateral and dorsal images of wild-type (WT), heterozygous (cdkl5^+/−), and homozygous (cdkl5⁻/⁻) mutant embryos with 3 dpf and 5 dpf. Scale bar = 0.5 mm. (C) Morphometric analysis of 5 dpf WT (n = 51), cdkl5^+/− (n = 50) and cdkl5^−/− (n = 71) embryos. Data are presented as median with interquartile range. Three independent experiments were performed. Statistical analysis was performed using Kruskal–Wallis followed by Dunn multiple comparisons test. *, **, ***, **** indicate p < 0.05, p < 0.01, p < 0.001 and p < 0.0001, respectively. ns indicates not significant. TL-total length; HL-head length; AHW-anterior head width; PHW-posterior head width.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cdkl5^{sa21938/sa21938} (AB) cdkl5^sa21938/+ (AB)
Observed In:	head head anterior region head posterior region whole organism
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.