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Morpholinos designed to deplete additional proteins involved in heterochromatic repression reactivate expression from the is7 transgene array. (A–D) Representative brightfield (left) and fluorescence (right) images of is7/+ transgenic larvae at 3 dpf. (A) Uninjected control, (B) larva injected with 4 ng of Cbx5 morpholino at the one-cell stage, (C) larva injected with 4 ng of Zbtb24 morpholino at the one-cell stage, (D) larva injected with 4 ng of Suv420h2 morpholino at the one-cell stage. (MO = morpholino). (E) Percent larvae injected with each morpholino that show high, medium or low dsRed fluorescence at 3 dpf compared to sibling uninjected controls (U = uninjected). n = ∼30 embryos per condition (F) Mean fluorescent intensity of larvae showing the highest dsRed fluorescence in morpholino injected larval pools, compared to uninjected sibling control pools at 3 dpf. All scale bars indicate 1 mm. Error bars indicate standard deviation.
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