FIGURE

FIGURE 1

ID

ZDB-FIG-220402-13

Publication

Calvird et al., 2022 - Uncovering Regulators of Heterochromatin Mediated Silencing Using a Zebrafish Transgenic Reporter

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FIGURE 1

The is7 transgene recapitulates key features of constitutive heterochromatin. (A–B) Brightfield (top) and fluorescence (bottom) images of representative wild-type (A) and is7/+(B) larva at 3 days post-fertilization (dpf). Scale bar indicates 1 mm. (C–D) Levels of the histone modifications H3K9me3, H4K20me3 and H3K27me3 at the is7 transgene array (C) or Sat1 pericentromeric repeats (D) as assessed by chromatin immunoprecipitation quantitative PCR (ChIP-qPCR). (E) 5-methycytosine (5 mC) enrichment (filled circles) at the is7 transgene array in two representative larval pools as detected by sodium bisulfite sequencing. Error bars indicate standard deviation.

Expression Data

Gene:	RFP
Fish:	is7Tg (AB)
Anatomical Term:	whole organism cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	is7Tg (AB)
Observed In:	whole organism histone H3K9 methyltransferase activity whole organism histone H3K27 methyltransferase activity whole organism histone H4K20 methyltransferase activity
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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