FIGURE

Fig. 3.

ID

ZDB-FIG-220305-17

Publication

Baronio et al., 2021 - Abnormal brain development of monoamine oxidase mutant zebrafish and impaired social interaction of heterozygous fish

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Fig. 3.

The dopaminergic and histaminergic systems of mao mutants. (A,B) Ventral views of whole-mount 10 dpf mao^+/+ (A) and mao^−/− (B) larval brains, anterior to the left, processed for vmat2 RNA ISH. n=4 for each genotype. (C) Bar charts showing results from RT-qPCR analysis of vmat2 in larvae of the indicated genotype at 10 dpf. n=4 for each genotype. (D-F) Ventral views of whole-mount 10 dpf mao^+/+ (D), mao^+/− (E) and mao^−/− (F) larval brains, anterior to the left, processed for tyrosine hydroxylase 1 (Th1) immunostaining. n=5 for each genotype. (G-I) Ventral views of whole-mount 10 dpf mao^+/+ (G), mao^+/− (H) and mao^−/− (I) larval brains, anterior to the left, processed for histamine (HA) immunostaining. n=5 for each genotype. (J,K) Bar charts showing results from Th1- and histamine-immunoreactive cell counting in larval brains of the indicated genotype at 10 dpf. (L,M) Bar charts showing results from RT-qPCR analysis of th1 (L) and hdc (M) in larvae of the indicated genotype at 10 dpf. Data are mean±s.e.m. One-way ANOVA followed by Tukey's post hoc test was used for statistical analysis. *P<0.05, **P<0.01, ***P<0.001. Scale bars: 75 μm.

Expression Data

Genes:	hdc slc18a2 th
Antibodies:	Ab1-histamine Ab1-th
Fish:	WT mao^{sa31732/sa31732} mao^sa31732/+
Anatomical Terms:	brain brain ventral region whole organism
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mao^{sa31732/sa31732}
Observed In:	brain ventral region whole organism
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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