FIGURE

Figure 1

ID

ZDB-FIG-220225-10

Publication

Paulissen et al., 2022 - Somite morphogenesis is required for axial blood vessel formation during zebrafish embryogenesis

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Figure 1

Retinoic acid (RA) is required prior to segmentation for angioblast migration.

(A–D) Dorsal view of tg(kdrl:eGFP) embryos with representative images taken every 60 min over the course of 180 min, in (A) untreated (N = 12, see Video 1), (B) aldh1a2-/- mutants (N = 12, see Video 2), (C) DMSO-treated (N = 10), or (D) 0.1 µM RA-treated embryos (N = 8, see Video 3). The anterior of the embryo is at the top, and red arrows indicate the midline. (E, F) Graph of fluorescence intensity as angioblasts arrive at the midline for (E) wild-type and aldh1a2-/- and for (F) DMSO and 0.1 μM RA. Y-axis indicates the percentage of fluorescent intensity at midline relative to the fluorescence intensity of whole embryo. Time is measured on the X-axis. N = 8 for all conditions, error bars indicate standard deviation. (G–I) Time-lapse images of tg(kdrl:eGFP) embryos treated with either (G) DMSO vehicle (n = 10) or (H) 20 μM DEAB at the five-somite stage (n = 6), and (I) 20 μM DEAB at tailbud stage (n = 14). Scale bars, 50 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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