PUBLICATION

Somite morphogenesis is required for axial blood vessel formation during zebrafish embryogenesis

Authors
Paulissen, E., Palmisano, N.J., Waxman, J., Martin, B.L.
ID
ZDB-PUB-220210-5
Date
2022
Source
eLIFE   11: (Journal)
Registered Authors
Martin, Benjamin, Waxman, Joshua
Keywords
cell biology, developmental biology, zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Embryo, Nonmammalian/blood supply*
  • Embryonic Development/physiology*
  • Gene Expression Regulation, Developmental/drug effects
  • Neovascularization, Physiologic/physiology*
  • Retinoids/pharmacology
  • Somites/physiology*
  • Tretinoin/metabolism
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • p-Aminoazobenzene/analogs & derivatives
  • p-Aminoazobenzene/pharmacology
PubMed
35137687 Full text @ Elife
Abstract
Angioblasts that form the major axial blood vessels of the dorsal aorta and cardinal vein migrate towards the embryonic midline from distant lateral positions. Little is known about what controls the precise timing of angioblast migration and their final destination at the midline. Using zebrafish, we found that midline angioblast migration requires neighboring tissue rearrangements generated by somite morphogenesis. The somitic shape changes cause the adjacent notochord to separate from the underlying endoderm, creating a ventral midline cavity that provides a physical space for the angioblasts to migrate into. The anterior to posterior progression of midline angioblast migration is facilitated by retinoic acid induced anterior to posterior somite maturation and the subsequent progressive opening of the ventral midline cavity. Our work demonstrates a critical role for somite morphogenesis in organizing surrounding tissues to facilitate notochord positioning and angioblast migration, which is ultimately responsible for creating a functional cardiovascular system.
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Human Disease / Model
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Mapping