The hepatomegaly of lvh is caused by mutation of ftcd. (A) Sequencing of ftcd from the wild-type and lvh mutant identified a G to A point mutation, resulting in a switch from glycine to arginine at position 691. Red arrow indicates the mutation site. (B) Diagramed FTCD protein structure and the mutation in lvh at FT domain C-subdomain. (C) A conserved β8 motif of FTCD in zebrafish and its homologous proteins in humans, mouse and chicken. Asterisk indicates the site of mutated amino acid in lvh. (D) WISH images showing ftcd expressions at 48 hpf, 72 hpf, 4 dpf, and 5 dpf. (E) 3D confocal projection and 2D single-optical section images showing the liver of wild-type, lvh and, ftcdcq108 at 9 dpf. Higher magnification images of single hepatocytes are displayed. (F) Unpaired Student’s t-test for single hepatocyte volume in the wild-type (n = 5), lvh (n = 5) and cq108 (n = 5). (G) 3D confocal projection and 2D single-optical section images showing the liver of WT, lvh and lvh under cq110 background at 8 dpf. Higher magnification images of single hepatocytes are displayed. (H) Unpaired Student’s t-test for single hepatocyte volume in the wild-type (n = 5), lvh (n = 5) and lvh under cq110 (n = 5). Asterisks indicate statistical significance. ****P<0.0001. Data are represented as mean±SD. WT, wild-type. Scale bars, 50 μm.
|
