Figure 2

(A) Quantitative RT-PCR analysis of CM molecular markers. n = 3 biological replicates; 2 tailed Student’s t test. (B) Representative echocardiography images of WT controls and vmhcl^e13/+ mutants at diastole and systole. (C) Quantification of cardiac function indices measured using echocardiography in the vmhcl^e13/+ mutant and WT control at 8 months. n = 9; data are presented as the mean ± SD; unpaired 2 tailed Student’s t test. (D) Representative images of isolated hearts and quantification of the VSA normalized to the BW of fish at 8 months. n = 7; unpaired 2 tailed Student’s t test. (E) Representative images of H&E staining in the apex area and quantification of trabecular muscle density in fish at 8 months. n = 6; unpaired 2 tailed Student’s t test. (F) Maximum swimming speed of fish at 8 months. n = 9; 2-way ANOVA. (G) Kaplan–Meier survival curves of vmhcl mutant fish and WT controls. n = 25–27; log-rank test; data are presented as the mean ± SD. Scale bars: 2 mm in D and 300 μm in E.