Fig. 4

AFB1 alters the distribution of neutrophil granulocytes and modulates the inflammatory response to a local injury in zebrafish larvae. (A) Representative images of 120 hpf neutrophil-reporter Tg(mpx:EGFP) larvae after embryonic, sub-lethal exposure to AFB1. Scale bar = 500 µm. (B) The number of neutrophils counted in the abdominal region of the control and AFB1-exposed larvae (n = 3 replicates, n = 10 larvae per replicates). (C) Flow cytometric detection of mpx:EGFP+cells per 100.000 cells in control and AFB1-exposed larvae (n = 6 replicates, n = 15 pooled larvae per replicate). (D) Schematic figure of the tail fin transection model and the representative images of the transected fins of control and AFB1-exposed Tg(mpx:EGFP) larvae at 4 h post injury. After tail fin transection, neutrophils started to migrate to the site of injury. Scale bar = 500 µm (E) The graph showing the number of neutrophils at the wound site at 4 h and 12 h post injury (n = 5 replicates, n = 10 larvae per replicate). Data represent the mean and SD. “*” indicates statistical significance at p < 0.05 vs. the control (DMSO).