Fig. 1

ubtor is expressed in spinal interneurons and ubtor mutants show increased spontaneous coiling. A ubtor expression (upper) and EGFP expression (lower) in 28-hpf Tg(ubtor:GAL4;UAS:EGFP) zebrafish embryos (scale bar, 250 μm). B Expression and distribution of the ubtor gene in zebrafish embryo spinal cord tissues at 24, 28, and 32 hpf (dotted lines, spinal cord tissue from hindbrain to somite 20. Scale bar, 60 μm. C EGFP expression in the spinal cord tissue of embryos at somites 4–10 of the Tg(ubtor:GAL4;UAS:EGFP) line (scale bar, 20 μm). D Frequency of coiling movements of embryos inside the chorion of 28-hpf ubtor mutants and the wild-type (results from five biological repeats, N_WT = N_ubtor^−/− = 126, t₁₉₈ = 8.097). E Frequency of coiling movements outside the chorion of 28-hpf ubtor mutants and the wild-type (two biological repeats, N_WT = N_ubtor^−/− = 20, t₃₇ = 3.914). Values are represented as the mean ± SEM in D and E. ***P <0.001; P, posterior; A, anterior; D, dorsal; V, ventral.