Bort acutely affects MT behavior in DRG neurons in vivo. (A) Still images from live imaging of mCherry in the peripheral nerve fibers of zebrafish treated with 1.3 μM Bort for the indicated times. Each image includes endings innervating the surface of a scale. Scale bar, 100 μm. (B) Representative kymographs at 0 and 6 h of EB3-GFP comets in the peripheral nerve fibers of zebrafish treated with Bort. (C) EB3-GFP time course analysis of MT dynamics parameters in the peripheral nerve fibers of zebrafish treated as in B for the indicated times. Data were pooled from three individual fish containing four to five neurites with up to 80 to 220 comets. (D) Micrographs from third-instar Drosophila larvae fed with vehicle control (n = 7 animals, n = 28 cells) or 20 μM Bort (n = 7 animals, n = 45 cells) starting 24 to 28 h after egg laying. ppk-CD4-tdGFP was used to visualize ddaC nociceptive neurons. (E) Quantification of dendrite degeneration treated as in D. Each point refers to an averaged value of degeneration index in each animal (4 to 6 cells per animal). Bars, 50 µm. (F) Representative kymographs of EB1-GFP comets in control and nociceptive neurons of Drosophila larvae fed with Bort for 3 and 6 h. Scale bar, 10 μm. (G) EB1-GFP time course analysis of MT dynamics parameters in nociceptive neurons of larvae treated for the indicated times. Data are from up to 400 comets and 19 to 45 neurites for each group. Control data are pooled from vehicle controls (3 to 6 h). Data in C, E, and G are medians plus interquartile range, and statistics were analyzed by Mann–Whitney U test. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
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