FIGURE

Fig 3

ID

ZDB-FIG-211122-25

Publication

Kikel-Coury et al., 2021 - Identification of astroglia-like cardiac nexus glia that are critical regulators of cardiac development and function

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Fig 3

Adult gfap⁺ cells localize with neurons and synapses and impact neuronal development.

(A) Confocal 10 μm z-projection from adult Tg(gfap:gfp) heart stained with AcTub. Axons have primary (white arrowhead), secondary (purple arrowhead), and tertiary (gray arrowhead) branches. (B) Quantification of colocalization between primary, secondary, and tertiary branches of axons and gfap⁺ cells. (C) Top: confocal 10 μm z-projection of adult Tg(gfap:gfp) heart stained with AcTub and rotated 90°. Bottom: 3D rendering of the AcTub and GFAP (white dotted box) are presented below. The gfap⁺ cells associate with axons (white arrowheads). (D) Representative quantification of intensity over distance of AcTub and GFAP. (E) Top: confocal maximum z-projection of adult Tg(gfap:gfp) ventricles stained with SV2 or Synaptotagmin. Bottom: 90°-rotated 3D rendering of contact points (white arrowheads) between GFAP and SV2 or Synaptotagmin. (F) Quantification of Tg(gfap:nsfb-mCherry⁺) intensity in the heart or retina (location control) after injection of DMSO or MTZ. (G) Quantification of axonal number after MTZ or full laser ablation of nucGFP⁺ cells. (H) Confocal maximum z-projection of ventricle (white dotted outline) of 6 dpf Tg(gfap:nucGFP) with either MTZ injection or full laser ablation of nucGFP⁺ cells and stained with AcTub. Arrows indicate increased innervation and arrowheads indicate nucGFP⁺ cells. Data are represented as mean ± SEM. Scale bar equals 10 μm. Statistics summarized in S1 Table. See S1 Data for raw data. AcTub, acetylated tubulin; dpf, days postfertilization; MTZ, metronidazole; V, ventricle.

Expression Data

Genes:	EGFP GFP
Antibodies:	Ab-SV2 Ab1-tuba znp-1
Fish:	mi2001Tg mi2004Tg mi2004Tg; scz059Tg
Conditions:	chemical treatment by injection: metronidazole ablation: glial cell (sensu Vertebrata)
Anatomical Terms:	cardiac ventricle axon cardiac ventricle glial cell (sensu Vertebrata) cardiac ventricle synapse
Stage Range:	Day 6 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mi2004Tg mi2004Tg; scz059Tg
Conditions:	chemical treatment by injection: metronidazole ablation: glial cell (sensu Vertebrata)
Observed In:	cardiac ventricle cardiac ventricle axon cardiac ventricle glial cell (sensu Vertebrata) cardiac ventricle innervation retina retina axon
Stage:	Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.