FIGURE

Figure 5

ID

ZDB-FIG-211029-88

Publication

Athapaththu et al., 2021 - Gamma-Aminobutyric Acid (GABA) Promotes Growth in Zebrafish Larvae by Inducing IGF-1 Expression via GABA_A and GABA_B Receptors

Other Figures

All Figure Page

Back to All Figure Page

Figure 5

Inhibition of GABA_A and GABA_B receptors downregulates GABA-mediated IGF-1 and IGF-1R expression in preosteoblast MC3T3-E1 cells. MC3T3-E1 cells (1 × 10⁴ cells/mL) were treated with 5 µM each of (A,D) bicuculline, (B,E) CGP 46381, and (C,F) TPMPA 2 h before treatment with 10 mM GABA for seven days. Total RNA was extracted, and RT-PCR was performed to determine the expression level of IGF-1 and IGF-1R. Glyceraldehyde 3-phosphate dehydrogenase gene (GAPDH) was used as an internal control. The expression of IGF-1 and IGF-1R relative to that of GAPDH level was determined (bottom). In a parallel experiment, cell-free supernatants were collected, and ELISA was conducted. Significant differences among the groups were determined using Student’s t-test. All data are presented as mean ± standard error of the mean (*** p < 0.001). N.S., non-significant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.