FIGURE

Fig. 5

ID

ZDB-FIG-211029-102

Publication

D'Gama et al., 2021 - Diversity and function of motile ciliated cell types within ependymal lineages of the zebrafish brain

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Fig. 5

foxj1a and foxj1b play diverse roles in the formation of motile ciliated cells

(A) Lack of cilia in the dorsal telencephalon in foxj1b mutant (middle; n = 9) and throughout the larval ventricular system in double foxj1a/b mutant (n = 6) at 4 dpf. Maximum projection at two different depths is shown.

(B1 and B2) MCCs (arrowhead) are not affected in the foxj1b mutant on the 1-month dorsal telencephalon (B1; n = 6) and ChP (B2; n = 6). Cilia remain absent in the monociliated cells (arrow) located laterally to the MCCs as shown in the magnified inset.

(C1–C3) MCCs are not affected in the adult medial TC (C1; n = 3), ChP (C2; n = 4), and midline of the telencephalon/diencephalon (C3; n = 3). (C2) Monociliated cells are particularly affected in the ChP.

(D1–D3) Loss of cilia in the medial TC (D1) and ChP (D2) of adult gmnc;foxj1b double mutants (n = 4). (D3) Single cilia remained in the telencephalic midline of double mutants (arrow) (n = 4).

(E) Diverse genetic programs instruct the formation of ciliated cells in the TC/ChP and in the telencephalon midline.

TeO, optic tectum; sco, sub-commissural organ. Arrows indicate monociliated cells, arrowheads indicate MCCs, and asterisks indicate axon tracts. Cilia loss is indicated by hashtag symbols.

See also Figures S4 and S5.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	foxj1b^{sq5719/sq5719} foxj1a^{sq5717/sq5717}; foxj1b^{sq5719/sq5719} gmnc^sq34/sq34; foxj1b^{sq5719/sq5719}
Observed In:	brain motile cilium choroid plexus choroid plexus motile cilium choroid plexus multi-ciliated epithelial cell ciliated cell dorsal telencephalon single ciliated epithelial cell tela chorioidea motile cilium tela chorioidea multi-ciliated epithelial cell
Stage Range:	Day 4 to Adult

Phenotype Detail

Acknowledgments

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