FIGURE

Fig 3

ID

ZDB-FIG-211010-24

Publication

Phatak et al., 2021 - Grhl3 promotes retention of epidermal cells under endocytic stress to maintain epidermal architecture in zebrafish

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Fig 3

Whole mount RNA <italic toggle='yes'>in situ</italic> hybridization (WISH) reveals spatiotemporal association between cell rounding, <italic toggle='yes'>grhl3</italic> expression, and expression of cell adhesion genes.

Bright field images of control morphant (A) and myoVb morphant (B) embryos at 24, 30, 36, 48, and 72 hpf stained for grhl3 transcripts. Zoomed in images of dorsal head epidermis of control morphant (C) and myoVb morphant embryos (D) stained for grhl3 expression at 48 hpf. The grhl3 expression was observed near the epidermal regions showing cell rounding (black arrowheads). WISH reveals increased expression of cell adhesion genes, cdh1, cldn7b, cldne, oclna, and dsc2l, in and around the epidermal regions containing rounded cells in myoVb morphants at 48 hpf (E).

Expression Data

Genes:	cdh1 cldn7b cldne dsc2l grhl3 oclna
Fish:	TU + MO1-myo5b
Knockdown Reagent:	MO1-myo5b
Anatomical Term:	epidermis
Stage Range:	Prim-25 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + MO1-myo5b
Knockdown Reagent:	MO1-myo5b
Observed In:	epidermis
Stage Range:	Prim-25 to Long-pec

Phenotype Detail

Acknowledgments

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