FIGURE

Figure 5

ID

ZDB-FIG-210915-54

Publication

Zhu et al., 2021 - Loss of Ift74 Leads to Slow Photoreceptor Degeneration and Ciliogenesis Defects in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Figure 5

Opsin transport analysis in ift74 mutant. (A–D) Representative confocal images of transverse cryosections through the central retinae of wild-type and ift74 mutant larvae at 4 and 24 h after heat-shock induction. The subcellular distribution of the GFP-CT44 (green) was evaluated. Sections were counterstained with phalloidin (red) to visualize the outer limiting membrane and the outer plexiform layer. Cell nuclei were stained with DAPI in blue. (E) Relative GFP fluorescence intensity in the cell bodies measured between the outer limiting membrane and the outer plexiform layer. Asterisks indicate outer segments; arrowheads indicate the outer limiting membrane and arrows indicate the outer plexiform layer. Scale bar: 10 µm. **** p < 0.0001, ns, no significant.

Expression Data

Gene:	GFP
Fish:	ouc2001Tg ift74^{ouc2031/ouc2031}; ouc2001Tg
Condition:	heat shock
Anatomical Terms:	eye photoreceptor cell cell body eye photoreceptor cell photoreceptor inner segment eye photoreceptor cell photoreceptor outer segment
Stage Range:	Day 5 to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ouc2001Tg ift74^{ouc2031/ouc2031}; ouc2001Tg
Condition:	heat shock
Observed In:	eye photoreceptor cell cell body eye photoreceptor cell opsin transport eye photoreceptor cell photoreceptor inner segment
Stage Range:	Day 5 to Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.