FIGURE

Figure 1

ID

ZDB-FIG-210915-50

Publication

Zhu et al., 2021 - Loss of Ift74 Leads to Slow Photoreceptor Degeneration and Ciliogenesis Defects in Zebrafish

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Figure 1

michelin encodes a zebrafish homolog of the ift74 gene. (A,B) External phenotypes of wild-type (wt) and michelin mutants at 5 dpf. (C) Bar graph showing the percentages of embryos with body curvature defects in different groups as indicated. SP, splicing morpholino; ATG, translation-blocking morpholino. (D) Genetic map on LG 5 showing the number of recombinants near michelin/ift74 locus. The exon/intron structure of the ift74 transcript was shown in the middle. Bottom showing the sequence of wild-type and ift74 mutants generated with CRISPR/Cas9 methods. The sgRNA targeting site was indicated with overline, the PAM site was labeled in green and the changing bases in the target region were shown in red. (E) PCR analysis showing the amplification of ift74 ORF in 3 dpf wild-type, sibling, and michelin mutant larvae. (F–H) External phenotypes of 5 dpf ift74 mutant and morphant larvae as indicated. Scale bars: 500 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ift74^{ouc2030/ouc2030} ift74^{ouc2031/ouc2031} WT + MO2-ift74 WT + MO2-ift74 + MO3-ift74 ift74^ouc2031/+; ift74^ouc2030/+
Knockdown Reagents:	MO2-ift74 MO3-ift74
Observed In:	post-vent region
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.