Fig. 2

A Decreased lyz and mpx expression and decreased SB⁺ cells in asxl1 mutants. WISH for lyz, mpx, and SB staining (3 dpf; scale bar, 200 μm; black boxes show enlarged details; lyz WISH, asxl1^+/+, n = 16, asxl1^−/−, n = 17; mpx WISH, asxl1^+/+, n = 22, asxl1^−/−, n = 17; SB staining, asxl1^+/+, n = 19, asxl1^−/−, n = 24; two-tailed Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001; error bars, mean ± SD). qRT-PCR comparison of lyz and mpx expression between wild type and mutant (3 dpf larvae, n ≥ 10 per group, performed with four replicates; two-tailed Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001; error bars, mean ± SD). B May–Grünwald–Giemsa staining of neutrophils from 4 dpf embryos and quantification of mature neutrophils by morphology. Neutrophils were collected from over 400 embryos of each genotype asxl1^+/+ or asxl1^−/−. After staining, 100 cells were randomly chosen for further calculation. This process was repeated three times for statistical tests (scale bars, 10 μm; two-tailed Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001; error bars, mean ± SD). C Reduced lyz expression rescued by asxl1 expression. Left panel: WISH of lyz (3 dpf) after injection of asxl1 mRNA. Right panel: quantification of lyz⁺ cells in asxl1^+/+ and asxl1^−/− tails (0.1 ng mRNA/embryo; scale bar, 200 μm; black boxes show enlarged images; asxl1^sib, n = 19, asxl1^mut, n = 10, asxl1^sib R, n = 27, asxl1^mut = 6. R, mRNA injected; one-way ANOVA followed by LSD Fisher’s post hoc test, *p < 0.05, **p < 0.01, ***p < 0.001; error bars, mean ± SD).