FIGURE

FIGURE 8

ID

ZDB-FIG-210718-39

Publication

Hsiao et al., 2021 - The Incoherent Fluctuation of Folate Pools and Differential Regulation of Folate Enzymes Prioritize Nucleotide Supply in the Zebrafish Model Displaying Folate Deficiency-Induced Microphthalmia and Visual Defects

Other Figures

All Figure Page

Back to All Figure Page

FIGURE 8

The interplay between Mthfr and Mthfd1L. (A) FD larvae collected at the indicated stages were examined for the developmental timing-specific expression of mthfr during embryogenesis. (B) Wild-type embryos injected with mthfd1L cRNA or MO were examined for the expression of mthfr at 1 dpf. (C) FD embryos with/without formate supplementation were examined for mthfr mRNA levels at 2 dpf. (D) FD embryos with/without formate supplementation were examined for mthfd1L mRNA levels at 5 dpf. (E) Control and FD embryos supplemented with or without 5 mM formate were measured for their total folate content at 2 dpf. ***p-value < 0.001; **p-value < 0.01; *p-value < 0.05.

Expression Data

Genes:	mthfd1l mthfr
Fish:	WT WT + MO1-mthfd1l WT + MO2-mthfd1l cku1Tg
Conditions:	heat shock chemical treatment by environment: sodium formate, heat shock
Knockdown Reagents:	MO1-mthfd1l MO2-mthfd1l
Anatomical Term:	whole organism
Stage Range:	Prim-5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO1-mthfd1l WT + MO2-mthfd1l cku1Tg
Conditions:	chemical treatment by environment: sodium formate heat shock chemical treatment by environment: sodium formate, heat shock
Knockdown Reagents:	MO1-mthfd1l MO2-mthfd1l
Observed In:	whole organism whole organism folic acid
Stage Range:	Prim-5 to Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol