FIGURE

FIGURE 2

ID

ZDB-FIG-210526-16

Publication

Grivas et al., 2021 - Midkine-a Regulates the Formation of a Fibrotic Scar During Zebrafish Heart Regeneration

Other Figures

All Figure Page

Back to All Figure Page

FIGURE 2

Generation of mdka-KO zebrafish. (A) The mdka locus and DNA mutations introduced by CRISPR/Cas9. The red line indicates the CRISPER target site, red letters indicate insertions, and red hyphens deletions. (B) Mdka domain organization and predicted protein mutations. Red letters denote novel amino acids, red hyphen deletions. β stands for beta-strands and the hinge domain is shown in green. (C,D) Whole-mount ISH (WM-ISH) for mdka in 2-day post-fertilization (dpf) mdka^+/+ and mdka^cn105 embryos. Scale bar, 200 μm. (E) qPCR analysis of mdka and mdkb in 2 dpf mdka^+/+ and mdka^cn105 embryos. t-test; **P < 0.01; Mean ± SD. (F,G) Immunofluorescence staining of Mdka in 7 dpci mdka^+/+ and mdka^cn105 hearts. Scale bar, 100 μm. (H) Western blot analysis for Mdka in 7 dpci mdka^+/+, mdka^cn105, and mdka^+/cn105 hearts. Tub, a-Tubulin; kDa, kilodalton.

Expression Data

Genes:	mdka mdkb
Fish:	AB mdka^cn105/cn105 mdka^cn105/+
Condition:	cryoablation: cardiac ventricle
Anatomical Terms:	heart whole organism
Stage Range:	Long-pec to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB mdka^cn105/cn105 mdka^cn105/+
Condition:	cryoablation: cardiac ventricle
Observed In:	heart whole organism
Stage Range:	Long-pec to Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol