Figure 5

Viral signaling pathways are required for the gene expression and cell death phenotypes in uhrf1 mutants. (A) qPCR analysis of immune genes in uhrf1^−/− and dnmt1^−/− mutants compared to their WT siblings. Rplp0 is used as loading controls and the delta-delta Ct (DDCt) values were calculated by normalization to rplp0 and WT sibling controls for each individual clutch. Lines in the graph represents the median. Statistical significance is calculated by paired t-test. **p < 0.05, ***p < 0.005, ****p < 0.001. (B) qPCR performed on the livers from mavs^cr and sting^cr F₀ crispants in the uhrf1 and WT backgrounds to assess immune gene expression. DDCt is calculated after normalization of each gene to rplp0 and WT sibling controls for each clutch; this was performed for each crispants and for the not-injected embryos. Significance is calculated using 2-way Anova. *p < 0.05, **p < 0.01, ***p < 0.0005. (C) TUNEL analysis of uhrf1^−/− and phenotypically WT sibling livers at 5 dpf in tnfa^cr F₀ crispants and non-injected controls. Quantification of the number of TUNEL positive foci per total liver area for at least 3 livers per clutch in 4 clutches for each condition. Significance is measured by 2-way Anova test. **p < 0.05, ****p < 0.0001.