FIGURE

Fig. 7

ID

ZDB-FIG-210326-7

Publication

Koopman et al., 2021 - The zebrafish grime mutant uncovers an evolutionarily conserved role for Tmem161b in the control of cardiac rhythm

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Fig. 7

Ca²⁺ transients are prolonged in tmem161b zebrafish mutants. (A) Schematic of the GCaMP6f biosensor used to measure in vivo, cardiomyocyte-specific Ca²⁺ transients. (B) Ca²⁺ transient parameters. (C–E) Representative overlaid Ca²⁺ transients from the (C) atrium, (D) AVC, and (E) ventricle of tmem161b+/+ and tmem161b^−/− embryos. (F–H) Ca²⁺ transient parameters in 3 dpf tmem161b^+/+, tmem161b^+/−, and tmem161b^−/− embryos. (F) Transient upstroke time, showing a faster upstroke in the ventricle of tmem161b^−/− embryos. (G) Normalized GCaMP6f signal amplitude, demonstrating a higher ventricular Ca²⁺ transient amplitude in tmem161b^−/−. (H) Ca²⁺ recovery time, demonstrating a slower reuptake/efflux of Ca²⁺ in tmem161b^−/− ventricles (mean ± SD; n = 12 to 23; *P < 0.05, **P ≤ 0.01, ***P ≤ 0.001; n.s., nonsignificant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tmem161b^uq4ks/uq4ks; ccu1Tg; hu6531Tg
Observed In:	cardiac ventricle release of sequestered calcium ion into cytosol cardiac ventricle sequestering of calcium ion
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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