FIGURE 4

More severe vascular malformation in neuroserpin deficient zebrafish under CoCl₂ induced hypoxic injury. (A) Image of vessels of Tg (Fli1: EGFP) and Tg (serpini1^−/−-Fli1: EGFP) with or without 10 mM CoCl₂ induced injury. Tg (Fli1: EGFP) and Tg (serpini1^−/−-Fli1: EGFP) show normal vascular morphology in brain region including central artery (CtA), primordial hindbrain channel (PHBC), and primary head sinus (PHS). CoCl₂ caused vascular malformation both in Tg (Fli1: EGFP) and Tg (serpini1^−/−-Fli1: EGFP) under 10 mM CoCl₂-induced injury. More reduced vascular branches in brain area were observed in Tg (serpini1^−/−-Fli1: EGFP) group compared to Tg (Fli1: EGFP). Scale bar:100 um. White arrow indicates brain vessels. Dash white square box shows reduction of normal vessels in CoCl₂ treated groups. (B) Quantification of average fluorescence intensity of vessels in brain area in Tg (Fli1: EGFP) and Tg (serpini1^−/−-Fli1: EGFP) under 10 mM CoCl₂ induced injury. The data are presented as the mean ± SEM, n = 6 per group. Statistical analyses were performed with Student’s t-test, ***p < 0.001, ns: no significant, compared with WT in the same CoCl₂ concentration.