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Fig. 4

ID

ZDB-FIG-210315-4

Publication

Hilbert et al., 2021 - Transcription organizes euchromatin via microphase separation

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Fig. 4

Transcription onset locally reorganizes euchromatin.

a Representative simulated time-lapse of transcription onset (Transcription), RNA accumulation (RNA), and euchromatin organization (DNA) in the vicinity of a single transcriptionally activated chromatin chain. The same results were obtained in 12 simulations. b Radial analysis, starting at the time when transcription foci were first detected. The range indicates the radial distance from the centroid of a given transcription focus. Analysis averaged over 12 simulations. c Representative spinning disk confocal microscopy time lapse of elongating RNA polymerase II (Pol II Ser2Phos, visualized with phospho-specific antibody fragments) and DNA (SiR-DNA). Images show nuclear mid-sections of a single nucleus taken from zebrafish embryonic cell culture corresponding to the late blastula (sphere) stage. The same results were obtained in three independent experiments. d Radial analysis averaged over time-lapse recordings from 13 nuclei.

Expression Data

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Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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