Fig 5

(A) Volcano plot of proteins identified by tandem mass tag mass spectrometry (TMT-MS) from wild-type and ski7^-/- mutant embryos at 4 hours post-fertilization. Significantly up- and down-regulated proteins are coloured in orange and blue, respectively. Proteins associated with oxidation-reduction processes are highlighted (green circle) and their name is displayed. Proteins, whose RNA was also identified as differentially expressed based on RNA-seq (DEGs), are highlighted in bold. (B) Ski7^-/- mutant embryos show increased resistance to DTT-treatment. Representative images of the development of wild-type and ski7^-/- embryos incubated in the absence (untreated) or presence of 0.2 mM DTT. The red box highlights the time-frame during which wild-type embryos show the most obvious aberrant morphogenesis. (C) Quantification of developmental abnormalities upon treatment with 0.2 mM DTT during embryogenesis (hpf, hours post-fertilization). Each dot represents the quantification of an independent experiment (* = p-adjusted < 0.05; ** = p-adjusted < 0.01 from Kruskal-Wallis with Dunn’s multiple comparison test compared to WT untreated on each time point).