FIGURE

Fig. 4

ID

ZDB-FIG-210311-11

Publication

Castellanos et al., 2021 - Knockdown of hspg2 is associated with abnormal mandibular joint formation and neural crest cell dysfunction in zebrafish

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Fig. 4

dlx2a expression is normal in morphants. a-c Whole mount in situ hybridization (ISH) was performed to detect the expression of dlx2a at the Prim-5 stage (N = 12 larvae in random control (RC) group, and N = 11 larvae in hspg2 morphant (MO) group). Embryos were injected as described in the methods section and subjected to ISH to detect dlx2a expression in the pharyngeal arches labeled by black arrows. d qPCR was performed to detect the expression of dlx2a. Total RNA was isolated from RC and hspg2 MO samples (N = 10 per group); error bars represent the standard deviation of technical replicates obtained from a pool of 10 embryos/group

Expression Data

Gene:	dlx2a
Fish:	AB + MO4-hspg2
Knockdown Reagent:	MO4-hspg2
Anatomical Terms:	pharyngeal arch whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO4-hspg2
Knockdown Reagent:	MO4-hspg2
Observed In:	neural crest cell development
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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