Fig. 8. MZcdx1b−/− mutants display normal visceral organ left-right patterning and left-side expression of spaw and pitx2c as well as normal KV cilia number and length. RNA in situ hybridization was conducted on wild types (WT) and MZcdx1b−/− mutants to evaluate expression patterns in the heart (A) or digestive organs (B) using myl7 (A) or gata6 (B) RNA probes at 48 hpf. Quantitative data show the percentages of embryos displaying different expression patterns of myl7 in the heart or gata6 in various digestive organs of wild types and MZcdx1b−/− mutants. RNA in situ hybridization was conducted on wild types and MZcdx1b−/− mutants to evaluate expression pattern of spaw in the LPM (C) or pitx2c in the gut (D) during 18–19 s or 19–22 s stages. Quantitative data show the percentages of embryos displaying different expression patterns of spaw in the LPM or pitx2c in the gut in wild types and MZcdx1b−/− mutants. E KV cilia were visualized by immunofluorescence staining using anti-acetylated tubulin (aat) antibody at 10 s. Quantitative data show similar numbers and lengths of KV cilia in wild types and MZcdx1b−/− mutants. Embryos (A–D) are shown in dorsal view. Scale bars (A–D) represent 100 μm; scale bar (E) represents 10 μm. Chi-squared test of independence was conducted to compare expression patterns of myl7 in the heart, gata6 in the digestive organs, spaw in the LPM or pitx2c in the gut, between wild types and MZcdx1b−/− mutants. Student’s t-test was conducted to compare KV cilia number or length between wild types and MZcdx1b−/− mutants. ns, not significant. a, atrium; g, gut; i, intestine; l, liver; lpm, lateral plate mesoderm; p, pancreas; v, ventricle.
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