Inhibition of cholesterol synthesis is associated with decreased expression of WNT target genes. (a) RNA was isolated from homozygous carriers of the Vu57 allele (Vu57) or wild type siblings (Sibling) at 30 hours post fertilization (HPF) and the relative expression of axin2, edn1, lef1, and ccnd1 were measured by quantitative real time PCR (qPCR). N = 8/group in two biological replicates for a total N of 16. *p < .05. (b) Wild type larvae were treated with vehicle control (DMSO), 2 μM atorvastatin (ATOR), 8 μM lonafarnib (lona), or 1.5 μM Ro‐48‐8071 at sphere stage. Total RNA was isolated from a pool of embryos (N = 14) and the relative expression of axin2 was measured by quantitative real time PCR (qPCR). (c) Embryos were treated with vehicle control (DMSO), 1, 2, or 3 μM Ro 48‐8071 at the sphere stage and total RNA was isolated at 30 HPF from a pool of embryos (N = 30) and the relative expression of axin2 was measured by qPCR. *p < .05. Error bars represent SD. (d). RNA was isolated from homozygous carriers of the Vu57 allele or Sibling wild type at 30 HPF and the relative expression of dlx2a was measured using qPCR. N = 8/group with two biological replicates for a total N = 16. Error bars represent SD. (e) sox10 expression was monitored in embryos treated with vehicle control (DMSO), 1, 2, or 3 μM Ro 48‐8071 at the 30 HPF (N = 30). Error bars represent SD. *p < .05
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