FIGURE

Fig. 3

ID

ZDB-FIG-210202-30

Publication

Reichmann et al., 2020 - The zebrafish histamine H3 receptor modulates aggression, neural activity and forebrain functional connectivity

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Fig. 3

Effects of histamine receptor H3 (hrh3) deletion on neuronal activation and neurotransmitter levels. A, Representative images depicting differences in rpS6 immunoreactivity between aggression‐exposed wild‐type (WT/MIA) and mutant fish (KO/MIA) in the telencephalon and hypothalamus. The selected counting areas are indicated in blue. Scale bar: 150 µm. B‐G, Number of ribosomal protein S6 (rpS6)+ cells in (B) the lateral and (C) medial zone of the dorsal telencephalic area (Dl and Dm), (D) the dorsal and (E) ventral nuclei of the ventral telencephalic area (Vd and Vv), (F) the caudal zone of the periventricular hypothalamus (Hc) and (G) the area around the posterior recess of the diencephalic ventricle (PR) in hrh3^−/− (KO) and corresponding wild‐type (WT) fish under basal conditions (CO) and after 1 h aggression exposure (MIA). n = 3‐4. Two‐way ANOVA followed by Tukey's post‐hoc test in case of a significant interaction term. Otherwise significant main effects are indicated in the graph. ^‡P < .001, ^†P < .01, *P < .05 (H and I) HPLC measurements of neurotransmitter and neurotransmitter metabolite levels in (H) the telencephalon and (I) diencephalon. n = 5‐7. Bonferroni‐corrected t tests.*P < .05 vs 5‐HIAA WT levels. Data are presented as mean ± SEM

Expression Data

Gene:	rps6
Fish:	WT hrh3^uol1/uol1
Condition:	stress
Anatomical Terms:	caudal periventricular hypothalamus dorsal nucleus of ventral telencephalon lateral zone of dorsal telencephalon medial zone of dorsal telencephalon posterior recess ventral nucleus of ventral telencephalon
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT hrh3^uol1/uol1
Condition:	stress
Observed In:	caudal periventricular hypothalamus diencephalon (3,4-dihydroxyphenyl)acetic acid diencephalon (5-hydroxyindol-3-yl)acetic acid diencephalon dopamine diencephalon homovanillic acid diencephalon serotonin dorsal nucleus of ventral telencephalon lateral zone of dorsal telencephalon medial zone of dorsal telencephalon posterior recess synaptic transmission, serotonergic telencephalon (3,4-dihydroxyphenyl)acetic acid telencephalon (5-hydroxyindol-3-yl)acetic acid telencephalon dopamine telencephalon homovanillic acid telencephalon serotonin ventral nucleus of ventral telencephalon
Stage:	Adult

Phenotype Detail

Acknowledgments

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